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Associate Scientist Cellularization

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Company :
United Therapeutics

Location :
Manchester, New Hampshire

Expiry Date :
Mon, 05 Apr 2021 23:59:59 GMT

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Description :
Job Details Full-timeEstimated: $56,000 – $81,000 a year2 hours ago Qualifications – Microsoft Excel – Aseptic technique – Mammalian cell culture – Cell culture – Histology – Doctoral degree – Master’s degree Full Job Description What we do About United Therapeutics United Therapeutics Corporation focuses on the strength of a balanced, value-creating biotechnology model. We are confident in our future thanks to our fundamental attributes, namely our commitment to quality and innovation, the power of our brands, our entrepreneurial culture and our bioinformatics leadership. We also believe that our determination to be responsible citizens having a positive impact on patients, the environment and society will sustain our success in the long term. We currently have five approved products on the market and a long-term mission of providing an unlimited supply of transplantable organs for those who need them. Our company was founded by an entrepreneur whose daughter was diagnosed with a life-threatening condition. She sought to find treatment options and a cure for her daughter and patients like her. We are founder-led, and relentless in our pursuit of ‘medicines for life’. We continue to research and develop treatments for cardiovascular and pulmonary diseases, pediatric cancers and other orphan diseases. How you’ll contribute Develop strategies aiding in cellularization of 3D printed scaffolds to generate functional tissue engineered lungs. Create, optimize, and implement analytical metrics that provide insight into the stability and function of printed scaffolding material. Provide expertise on lung tissue engineering methodologies and techniques required for to generate a cellularized 3D printed lung scaffold. Support the development of methods and protocols to produce cellularized 3D printed lung constructs which are suitable for pre-clinical and in-vitro functional evaluation. Key Job Responsibilities Lead and contribute to efforts to create, develop, and evaluate cellularized 3D printed bioscaffolds for lung tissue engineering Collaborate to help develop and independently execute experiments to support cellularization using iPSC-derived lung populations Perform cell differentiation, expansion, and functional assessment of iPSC-derived lung populations, for cellularization Perform cell and molecular biology assays to evaluate cellularization and tissue function Make recommendations and set new directions for the project based on data, experimental results, and feedback from 3D printing, scaffold design, and bioink development leads Routinely report and present hypothesis driven experimental results, data, and plans to other team members individually and at group meetings Routinely maintain critical materials and reagents needed to complete assigned experiments Record experimental details and findings in individual and collaborative electronic notebook entries, updated at minimum on a weekly basis Establish and maintain a deep understanding of current and emerging technology in lung cell biology, lung physiology, bioscaffold design and lung tissue engineering Access and utilize primarily literature sources to support and develop experimental plans Routinely meet 1:1 with managers to review results and progress Perform other duties as assigned For this role you will need Minimum Qualifications MS degree in Cellular, Developmental, Molecular Biology, or related discipline with 3+ years of relevant experience Track record of success (products and/or publications) in tissue engineering, cellularization of scaffolds design for tissue engineering applications Experience with Histology and immunostaining, qPCR, cytokine arrays, metabolic assays, ELISAs, and Western blots Experience with stem cell differentiation protocols and induced pluripotent stem cell culture Knowledge and professional use of Aseptic Technique and mammalian cell culture Knowledge and understanding of Stem cell differentiation and evaluation Experience with engineered reporter cell lines Strong comprehension of tissue engineering concepts and current field Knowledge of cell, extracellular niche, and biomaterials interaction Knowledge of and experience with Imaging and microscopy Knowledge of and experience with protein and molecular biology assays and techniques (histology, qPCR, ELISA, cytokine arrays, metabolic assays) Proficient with MS Office suite (Word, Excel, PowerPoint, Outlook) Preferred Qualifications PhD degree in Cellular, Developmental, Molecular Biology, or related discipline Knowledge of and experience with lung epithelial air-liquid interface (ALI) culture Experience with Prism GraphPad Experience with electronic notebooks Life as a Unitherian At United Therapeutics, you’ll realize quickly that it is not an ordinary place to work! When you join our company, you will learn, grow, contribute, have fun, and be challenged… all while making a difference in the lives of our patients. United Therapeutics Corporation is an Equal Opportunity/Affirmative Action Employer – EOE Minorities / Females / Protected Veterans / Individuals with Disabilities Show full job description Apply Now