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Neuroscience Research Assistant

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Company :
University of California – San Diego Medical Centers

Location :
San Diego, California

Expiry Date :
Fri, 16 Apr 2021 23:59:59 GMT

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Description :
UCSD Layoff from Career Appointment: Apply by 3/16/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Special Selection Applicants: Apply by 3/26/21. Eligible Special Selection clients should contact their Disability Counselor for assistance. DESCRIPTION =========== The Department of Neurosciences is a leading clinical-academic department within the UCSD School of Medicine and is comprised of more than 130 faculty members. The Department s mission is to create the synergies and define the ideas and programs that will allow us to transform the way our neurosciences community discovers, teaches and cares for patients. We strive to conduct cutting-edge scientific research and train the next generation of neurologists and researchers by fostering education at all levels and integrating the latest scientific and medical advances into the curriculum. Under supervision, the incumbent will assist in basic-translational research in the Developmental Neurosciences Research Program (DNRP) at UCSD in the Altman Clinical and Translational Research Institute. The individual will conduct independent research including performing a limited variety of highly specialized laboratory procedures in molecular and cell biology under the direction of the Laboratory Supervisor and Project Scientist. The main research themes are epilepsy, experimental therapeutics – in particular, metabolism-based treatments such as the ketogenic diet, neurodevelopmental disorders (especially, autism and attention deficit hyperactivity disorder), pediatric stroke and traumatic brain injury, brain inflammation. Primary responsibilities will include standard laboratory procedures with neuronal stem cells and mouse primary and organotypic slice cultures, performing stereotaxic electrode implantation for Video-EEG experiments in rodents, assisting in metabolic studies by conducting metabolic flux assays (e.g., with the Seahorse XFe96 flux analyzer) and other metabolism-based readouts and overseeing mouse colony husbandry, and collection of mouse tissues for genotyping. Will attend and present at lab meetings and contribute to the writing of grants and manuscripts for publications. Incumbent will also perform general lab duties including ordering supplies and equipment. MINIMUM QUALIFICATIONS ====================== * Theoretical knowledge of neuroscience, molecular biology, or closely related fields. * Demonstrated experience with tissue culture and knowledge of sterile techniques. * Demonstrated experience in handling mice and rats, minor and major stereotaxic surgery techniques, injections and tissue collection. * At least three years practical laboratory experience, such as supervised research project, independent study, or work experience in the Neuroscience area. * Experience or theoretic background in Bioenergetics and Mitochondrial assays. * Experience to perform required experiments independently, and troubleshoot as needed, with attention to detail, analyze results, and provide accurate documentation. * Strong computer skills using Microsoft Word, Excel, PowerPoint, email system, and a variety of web-based programs. Ability to create and maintain databases and spreadsheet software, perform standard computer tasks, and learn new programs/applications with minimal direction. * Experience with immunocytochemistry and microscopy techniques. * Knowledge and experience to perform standard and routine laboratory techniques such as, but not limited to, biochemical assays, DNA and RNA extraction and quantification, polymerase chain reaction (PCR), protein extraction and western blotting. * Highly developed organizational and time management skills, with the ability to keep track of various concurrent courses, ability to change direction with little or no notice and effectively prioritize work. * Excellent interpersonal skills including tact, diplomacy and flexibility with the ability to work in a laboratory environment in conjunction with principles of community with staff, faculty, researchers and students. Promote effective and respective interactions with a large number of research and technical personnel and other laboratories essential to foster and enliven research and academics. * Knowledge of basic lab safety requirements and procedures and EH&S regulations and methods for handling, clean-up, and waste disposal for biohazardous materials. * Ability to adhere to established UCSD guidelines for safety, animal care, and environmental issues. PREFERRED QUALIFICATIONS ======================== * Master’s degree preferred. * Experience with primary tissue and stem cell culture techniques. SPECIAL CONDITIONS ================== * Must be able and willing to work with mice and rats. * Employment is subject to a criminal background check. * Willingness to work with animals and standard hazardous laboratory compounds. * Willing to work evenings and weekends when necessary.