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Research Assistant II Lab

  • Anywhere

Company :
Lowell General Hospital

Location :
Boston, Massachusetts

Expiry Date :
Sat, 19 Dec 2020 23:59:59 GMT

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Description :
Working under general supervision of the laboratory#s Principal Investigator and his collaborator Dr. Robert Blanton will execute protocols involving a related to the fields of molecular biology, cellular biology, and cell signaling.# Works with the Principal Investigator in developing new protocols and modifications of existing techniques.# Records, calculates, transcribes and analyses data using statistical techniques.# Responsibilities: Under general supervision, executes protocols for research experiments.# Prepares nucleic acids and harvests tissue for analysis of gene expression, post translational modification of proteins by gel electrophoresis techniques (quantitative PCR and Western blotting); cGMP and ANP assays.# # Will be trained to generate high quality cardiomyocytes in culture and measure Ca transients, contractility in response to pacing protocols using Ionoptix technology. Will maintain and treat mice prior to harvesting of cardiomyocytes or treat cardiomyocytes with small molecule activators or inhibitors of signaling pathways prior to physiologic studies or harvesting for effects on second messengers and singling proteins. Records, calculates, transcribes and analyzes data using statistical techniques. Presents experimental data at laboratory meetings. Independently analyses experimental results and troubleshoots in discussion with the Principal Investigator. Participates in the design of new experiments.# Performs interlibrary literature searches. Maintains laboratory supplies and inventory. Works as part of a collaborative research team in which, cell signaling, calcium homeostasis and contractile state are assessed in cell culture systems as well as animal model systems (transgenic mice). Maintains established department policies, procedures, objectives, quality assurance, safety, environmental and infection control.# Performs all other duties and responsibilities as assigned.# Requirements: Bachelor of Science degree in a biological/physical science is highly recommended.# An associate degree in a biological/physical science with extensive work experience will also be considered. Minimum of two to three years progressive experience in research laboratory setting with cell culture experience. An equivalent combination of education and experience, which provides proficiency in the areas of responsibility listed above, may be substituted for the above education and experience requirements. Excellent problem-solving skills. Strong interpersonal skills and ability to work under pressure (time-sensitive studies and deadlines) as well as with other members of the research team. Must possess strong oral and written communication skills, attention to detail and reliability.# Highly organized and highly trustworthy and thus able to work with both expensive equipment and highly precious animals. Knowledge of general laboratory techniques including a sound foundation in basic cellular and molecular techniques. JobSummaryDisplay: Working under general supervision of the laboratory’s Principal Investigator and his collaborator Dr. Robert Blanton will execute protocols involving a related to the fields of molecular biology, cellular biology, and cell signaling. Works with the Principal Investigator in developing new protocols and modifications of existing techniques. Records, calculates, transcribes and analyses data using statistical techniques. Responsibilities: Under general supervision, executes protocols for research experiments. Prepares nucleic acids and harvests tissue for analysis of gene expression, post translational modification of proteins by gel electrophoresis techniques (quantitative PCR and Western blotting); cGMP and ANP assays. Will be trained to generate high quality cardiomyocytes in culture and measure Ca transients, contractility in response to pacing protocols using Ionoptix technology. Will maintain and treat mice prior to harvesting of cardiomyocytes or treat cardiomyocytes with small molecule activators or inhibitors of signaling pathways prior to physiologic studies or harvesting for effects on second messengers and singling proteins. Records, calculates, transcribes and analyzes data using statistical techniques. Presents experimental data at laboratory meetings. Independently analyses experimental results and troubleshoots in discussion with the Principal Investigator. Participates in the design of new experiments. Performs interlibrary literature searches. Maintains laboratory supplies and inventory. Works as part of a collaborative research team in which, cell signaling, calcium homeostasis and contractile state are assessed in cell culture systems as well as animal model systems (transgenic mice). Maintains established department policies, procedures, objectives, quality assurance, safety, environmental and infection control. Performs all other duties and responsibilities as assigned. Requirements: Bachelor of Science degree in a biological/physical science is highly recommended. An associate degree in a biological/physical science with extensive work experience will also be considered. Minimum of two to three years progressive experience in research laboratory setting with cell culture experience. An equivalent combination of education and experience, which provides proficiency in the areas of responsibility listed above, may be substituted for the above education and experience requirements. Excellent problem-solving skills. Strong interpersonal skills and ability to work under pressure (time-sensitive studies and deadlines) as well as with other members of the research team. Must possess strong oral and written communication skills, attention to detail and reliability. Highly organized and highly trustworthy and thus able to work with both expensive equipment and highly precious animals. Knowledge of general laboratory techniques including a sound foundation in basic cellular and molecular techniques.