Scientist / Senior Scientist, High-dimensional construct engineering

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Company :
Generation Bio

Location :
Cambridge, Massachusetts

Expiry Date :
Tue, 20 Jul 2021 23:59:59 GMT

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Description :
Generation Bio is an innovative genetic medicines company focused on creating a new class of non-viral gene therapy to provide durable, redosable treatments for people living with rare and prevalent diseases. The companys non-viral platform incorporates a proprietary, high-capacity DNA construct called closed-ended DNA, or ceDNA; a cell-targeted lipid nanoparticle delivery system, or ctLNP; and an established, scalable capsid-free manufacturing process. The platform is designed to enable multi-year durability from a single dose of ceDNA and to allow titration and redosing if needed, and our scalable manufacturing process supports our mission to extend the reach of gene therapy to more people, living with more diseases, in more places around the world. Our vision is to make the ravages of genetic disease as imaginary to the next generation as polio feels to children today.

We are a thriving, collaborative, creative community of 120 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.

Delve further. Please visit [ follow @generationbio [


Generation Bio is seeking an energetic and highly motivated molecular biologist(s) to expertise in the area of design, development and execution of High-throughput pooled genetic screens. As part of our Platform biology team, the scientist hired will apply these approaches to the identification of ceDNA constructs with engineered improvements in performance from large combinatorial libraries, as well as testing of design concepts to aid rationale design strategies. This position offers high visibility in the company and opportunity to play pivotal role in advancing our platform technology. The role is cross-functional, with key interactions with bioinformatics, manufacturing and pre-clinical groups.


* Design and development of high throughput massively parallel genetic screening approaches for optimizing ceDNA construct performance in mammalian cells
* Analysis of construct performance and molecular attributes in vitro
* Work with bioinformatics to develop and implement workflows for analysis of multidimensional data sets.
* Utilize modern synthetic biology approaches to develop and implement strategies for vector and library construction.
* Oversee and manage day-to-day activities of Research Associates
* Work with a cross functional team including pharmacology and bioinformatics

* Manage CRO activities, including project design, timelines and quality of deliverables


* PhD in Molecular Biology, Virology, Mammalian Cell Biology, Biochemistry or related discipline
* Minimum of 1 year of industry experience preferred
* Fluency and extensive hands-on experience with molecular biology techniques with a focus on application to mammalian systems
* Demonstrated hands-on expertise with concepts and techniques for execution of high dimensional massively parallel genetic screens.
* Experience with NGS methods and analysis, a plus
* Experience/ knowledge of AAV constructs, a plus
* Experience with bioinformatic analysis (e.g. Database curation/mining, Sequence analysis techniques and statistical evaluation of high-dimensional data sets), a plus

POSITION: Full-Time, Exempt

EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, gender, religion, national origin, gender identity, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.