Senior Staff Research Associate

  • Anywhere

Company :
University of California – San Diego Medical Centers

Location :
San Diego, California

Expiry Date :
Fri, 06 Nov 2020 23:59:59 GMT

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Description :
UCSD Layoff from Career Appointment: Apply by 10/7/20 for
consideration with preference for rehire. All layoff applicants should
contact their Employment Advisor.
Special Selection Applicants: Apply by 10/16/20. Eligible Special
Selection clients should contact their Disability Counselor for
The UCSD Department of Cellular and Molecular Medicine (CMM) is one of
two basic science units in the School of Medicine where world-class
researchers study fundamental cellular processes and pathways using a
variety of techniques from classical genetics and fluorescence
microscopy to high-throughput genomics, systems biology, and
crystallography. The Reck-Peterson lab at UC San Diego studies the
molecular mechanisms of intracellular transport, and how defects in
transport lead to neurodevelopmental and neurodegenerative disease.
The Reck-Peterson lab is funded by the National Institutes of Health,
the Howard Hughes Medical Institute, and Aligning Science Across
Parkinson’s (ASAP).
The Senior Laboratory Researcher will independently design and execute
experiments to study the cellular basis of Parkinson s Disease (PD).
Specifically, the lab studies Leucine Rich Repeat Kinase 2 (LRRK2),
the most commonly mutated gene in familial PD. The senior research
associate will use gene editing of standard tissue culture cell lines
as well as ES and iPS cells to generate a variety of cell models and
tools for the project, will use differentiation protocols for neurons
and macrophages, will use advanced live- and fixed-cell light
microscopy to image these cells, will use biochemistry including
proximity-dependent labeling and mass spectrometry, kinase assays and
GTPase assays to probe the cellular environment of LRRK2.
The Senior Laboratory Researcher will manage and coordinate research
efforts with other collaborators both within and outside the
laboratory. They will independently conceive, plan, and conduct
research projects based on reviews of scientific literature and recent
advances in related fields, organizing and executing all techniques
involved. They will contribute novel ideas of major methodological
significance to the research projects, design key experiments and
develop new techniques. They will author or co-author scientific
journal manuscripts and book chapters. They will direct and advise the
work of technicians, students, and post-doctoral fellows to enable
them to successfully participate in laboratory projects, as well as
present their findings at lab meetings and conferences, and perform
other duties as needed.
Advanced theoretical knowledge of intracellular transport,
neurodegenerative diseases, cell biology, biochemistry, and
molecular biology as typically achieved by a college degree, or an
equivalent combination of education and experience.
Strong demonstrated experience in CRISPR-mediated gene-editing and
experience in culturing, editing and differentiating human ES and
iPS cells.
Strong demonstrated experience in live- and fixed-cell imaging
using confocal microscopy.
Strong demonstrated experience in protein biochemistry and mass
spectrometry analysis.
Proven experience in designing, coordinating, executing, and
analyzing data.
Advanced skill in experimental design and adaptation of methods to
achieve results. Demonstrated experience in integrating data from
multiple sources to synthesize conclusions. Knowledge and ability
to read and abstract scientific articles and apply to the design
of experiments.
Demonstrated experience in the official review of manuscripts for
publication in peer-review journals.
Experience in providing technical work direction and training of
students, post-docs, and technicians.
Proven experience and knowledge in developing hypotheses, design
experiments, conduct experiments, analyze data, write manuscripts,
and publish results in peer-reviewed journals, including all
interactions with Reviewers and Editors.
Demonstrated experience in working with collaborators in other
universities on joint projects, and working under milestones
driven projects, including knowledge in writing reports.
Strong organizational skills, including experience in laboratory
record keeping, budget analysis and managing logistics.
Demonstrated knowledge of lab safety rules.
Proven experience in contributing and implementing innovative
ideas and techniques on complex and multi-disciplinary research
Proven ability to read scientific papers and adapt knowledge and
techniques from the literature to experimental systems.
Excellent written, verbal and interpersonal communication skills
including tack, diplomacy, and flexibility in order to work and
interact effectively with staff, researchers, and management in
person, on the phone or via Zoom or other video conferencing
Knowledge of PC software applications such as Word, Excel,
PowerPoint, Keynote, Illustrator to produce reports,
correspondence, graphs, and spreadsheets to generate, track, and
analyze data.
PhD in a field related to cell biology, biochemistry or
Experience with UCSD regulations, policies, and procedures.
Employment is subject to a criminal background check.
Occasional evenings and weekends may be required.
Must be willing to work with hazardous materials.