Technical Research Assistant I/ 40 Hours/ Genetics Sherwood Lab

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Company :
Brigham & Women’s Hospital(BWH)

Location :
Boston, Massachusetts

Expiry Date :
Mon, 08 Mar 2021 23:59:59 GMT

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Description :
Employer Name: SpiderID: Location: Boston, Massachusetts Date Posted: 2/4/2021 Wage: Negotiable Category: Law Enforcement/Security/Safety Job Code: -1427 Job Description: Working independently, under general supervision, performs cutting-edge research projects involving the use of CRISPR-Cas9 high-throughput screening to understand genetic disease and identify gene therapy strategies in the Sherwood lab ( This person will be in charge of all aspects of the projects for which they are responsible, which will include: protocol design and optimization; data generation using molecular cloning, cell culture techniques, and nextgen sequencing library preparation; data analysis; and writing scientific papers to communicate salient findings. A strong academic background in genetics and CRISPR-Cas9 is expected, and prior research experience at the undergraduate level or higher is required. While the primary responsibilities are wet lab experimental, some computational background is preferred. PRINCIPAL DUTIES AND RESPONSIBILITIES: Indicate key areas of responsibility, major job duties, special projects and key objectives for this position. These items should be evaluated throughout the year and included in the written annual evaluation. Performs experimental laboratory work in the field of genomics, stem cell biology, and genome editing. Full project management requires integrated performance of all of these tasks on a daily basis along with organizational skills to balance project demands. Work will include: – Molecular biology including devising and carrying out plasmid cloning strategies. – Cell culture including routine maintenance, genetic manipulation, and high-throughput CRISPR-Cas9 screening. – Genome editing-based screens including library production, testing, genomic library preparation and data analysis. – Genomic procedures including ChIP-seq, DNase-seq, single cell RNA-seq, PCR, and library preparation. – Creative employment of novel laboratory techniques as the project demands. Other responsibilities include: – Maintain basic records of methodologies and test results in a log of work performed – Identify unusual findings and report such findings to supervisor – Maintain equipment and supplies in a neat and orderly manner – Maintain established department policies, procedures, objectives, quality assurance, safety, environmental and infection control – Maintain established hospital policies, procedures, and objectives for laboratory safety Performs other duties and responsibilities as directed. Previously trained technicians have co-authored journal articles and moved on to MD and PhD programs, so we are looking for someone who could commit 2 years to an exciting research position to prepare them for graduate/medical school. To apply, send a cover letter, CV, and names and contact information for two references to . Qualifications – B.S. in a biological/physical science required. – Lab experience is required, experience with mammalian cell culture, molecular cloning, and genomic work is preferred. SKILLS/ ABILITIES/ COMPETENCIES REQUIRED: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.) – Must have a sound understanding of laboratory policies and procedures – usually acquired through one-three months on-the-job training. – Must have good manual dexterity. – Must demonstrate careful attention to detail. – Must be able to maintain a neat and orderly working environment. WORKING CONDITIONS: Normal research/molecular biology laboratory with exposure to radioactive and other biohazardous materials under controlled conditions. EEO Statement Brigham and Women’s Hospital (BWH) complies with applicable Federal civil rights laws and does not discriminate on the basis of race, color, national origin, citizenship, alienage, religion, creed, sex, sexual orientation, gender identity, age, or disability. BWH does not exclude people or treat them differently because of race, color, national origin, citizenship, alienage, religion, creed, sex, sexual orientation, gender identity, age, or disability. Partner’s Healthcare is acting as an Employment Agency in relation to this vacancy. Job Criteria: Start Date: Position Type: Full-Time Permanent Years of Experience Required: Education Required: Overnight Travel: Vacation Time: Contact Information: Contact Name: Partner’s Healthcare Company Type: Company: Brigham & Women’s Hospital(BWH) City: State: Zip: