Website Saviance Technologies
Saviance Technologies
Company : Saviance Technologies
As a Sr. Scientist in Immunology, you will support the development of Client allogeneic cell therapies by developing immune cell-based assays, executing in vitro experiments, and assessing immune response to different gene edited cells. You will contribute to the early-stage validation of gene edits and will work closely with cross-functional teams to advance cell therapy programs to preclinical development.
-Develop and optimize cell-based assays using primary human immune cells, such as mixed lymphocyte reaction, T-cell, NK-cell cytotoxicity assays, phagocytosis assays, etc.
-Isolate and culture different immune cells from either PBMCs or fresh whole blood using immunomagnetic isolation techniques.
-Perform differentiation of monocyte to macrophages or dendritic cells, as needed
-Assess immune checkpoint receptor expression using multi-parameter flow cytometry techniques
-Use different gene editing techniques, including CRISPR to create knockouts or transgenic cell lines
-Gene edit different cell types including iPSCs, primary cells, and immortalized cell lines
-Use different methods, including cell sorting, single cell cloning to create gene edited clonal cell lines
-Design and execute experiments and analyze resulting data to evaluate efficacy of gene edited cells in in vitro assays
-Communicate experimental data effectively through written reports and oral presentations.
-Demonstrate flexibility and motivation to learn and apply new technologies for biological assays.
Quals–
-Bachelor s degree in Immunology, Cell Biology, or a related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology.
-Master s degree in Immunology, Cell Biology, or a related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology.
Additional Skills/Preferences:
-Prior experience with using live cell analysis platforms such as Incucyte and Xcelligence will be a plus
– Proficient in developing in vitro assays, including work with human cell lines, PBMCs, and whole blood assays.
-Proven ability to utilize immune cell and cytokine analytical techniques, including multiplex ELISA, MSD and flow cytometry
-Prior work with CRISPR/Cas9 gene editing techniques.
-Prior work with cell culture; Primary and mammalian cell lines
– Strong analytical, problem-solving, and decision-making skills.
– Excellent interpersonal and communication skills.
-Ability to work independently and collaboratively within a team environment