Website CYNET SYSTEMS
CYNET SYSTEMS
Company : CYNET SYSTEMS
Job Description:
Pay Range: $45.76hr – $50.76hr
Responsibilities:
- Execute NGS library preparation (e.g., bulk RNA, scRNA, snRNA sequencing), quantification, and sequencing workflows to support the DOCTA team.
- Internalize and develop new NGS workflows to support evolving needs.
- Develop and adhere to standard operating procedures for workflows to ensure consistent production of high-quality data.
- Work in the sequencing space cross-functionally, with the ability to manage multiple requests and queues.
- Deliver NGS data with clear expectations of timelines to multiple teams and promptly update stakeholders of run completion.
- Effectively manage inventory of DNA, RNA, libraries, and sequencing reagents.
- Perform additional related tasks (i.e., RNA extraction, qPCR/ddPCR) as needed to help the team meet goals and deliverables.
- Maintain detailed documentation of NGS run performance and scientific work in an electronic notebook.
- Participate in assessment of sample prep and sequencing workflows at other sites (Indianapolis and/or contract research organizations) to enable flex capacity when demand is high (no travel required).
Qualifications:
- B.S. and/or M.S. in biological or biochemical sciences (or a related field) with 3-5 years of laboratory research experience.
- Molecular biology mastery of nucleic acid extraction and purification, quantification, NGS library preparation/QC, and qPCR.
- Strong background in NGS technologies and their applications, including quantification, sequencing, and data QC (preferably Illumina).
- Direct experience with RNA-seq (bulk) and amplicon-seq.
- Experience with single-cell and single-nuclei RNA-seq applications.
Additional Skills/Preferences:
- Familiarity with CRISPR-based gene editing and high-throughput screening is a plus.
- Experience with automation is a plus (Mantis, Integra systems, Liquid Handlers, SPTLabtech Firefly, Tecan, etc).
- Strong organizational, time management, and communication skills.
- Experience with documentation in electronic notebook software.