Scientist

Company : Sigma Inc

(phone number removed) Scientist  – Functional Genomics (CRISPR, Single-Cell, NGS)

Ridgefield, CT | Onsite Only | 12-Month Contract

Sigma Systems is seeking a highly experienced Scientist IV to join a cutting-edge Functional Genomics and Single-Cell Biology team in Ridgefield, CT. This is a strictly onsite role supporting advanced research in CRISPR-based functional genomics, next-generation sequencing, and single-cell technologies within a collaborative, multidisciplinary environment.

This position is ideal for a senior-level scientist who thrives in hands-on lab work, experimental design, and technical leadership.

Key Responsibilities

• Design, develop, and execute functional genomics assays, including CRISPR-based editing (RNP delivery, Perturb-seq)

• Perform single-cell sequencing, CITE-seq, and spatial transcriptomics workflows

• Prepare NGS libraries, including sample preparation, QC, and troubleshooting

• Provide technical guidance and training to research scientists on FGSL technologies

• Interpret experimental outcomes, propose follow-up experiments, and explore new research avenues

• Maintain detailed laboratory documentation in compliance with SOPs and policies

• Operate, maintain, and troubleshoot laboratory equipment

• Define and execute appropriate quality control (QC) measures

• Collaborate with cross-functional research teams to support project goals

• Contribute to protocols, technical reports, and written documentation

• Automate data processing, results reporting, and delivery where applicable

• Ensure data integrity, ethical research practices, and regulatory compliance

Required Qualifications

• Master’s degree in Molecular Biology, Cell Biology, or a related scientific discipline

• 4+ years of hands-on experience in molecular biology, functional genomics, or related fields

• Proven CRISPR expertise:

  • Personally designed guide RNAs

  • Performed transfections/infections

  • Selected edited cells

  • Validated knockouts/knockins

• Next-Generation Sequencing (NGS):

  • Hands-on library preparation experience (not analysis-only)

• Single-cell technologies:

  • Sample preparation

  • Library generation

  • Quality control

• Strong experience with cell culture, sterile technique, and transfections

• Proficiency in standard molecular biology techniques:

  • qPCR / RT-qPCR

  • Western blot

  • Molecular cloning

  • Gel electrophoresis

• Demonstrated ability to troubleshoot complex experiments

• Excellent oral and written communication skills

• Ability to work effectively in a highly collaborative, multidisciplinary environment

Preferred Qualifications

• Experience with automation or high-throughput workflows

• Ability to perform multiple functional genomics techniques in parallel

• Familiarity with emerging functional genomics and single-cell research methodologies

Apply Online