Scientist I

Company : Katalyst Healthcares & Life Sciences

Responsibilities:

• Perform routine and advanced wet lab protocols in cellular and molecular biology.
• Maintain and culture human stem cells and their derivatives (maintenance, passaging, freezing/thawing).
• Support cell culture operations such as media preparation, aliquoting reagents, and general lab upkeep.
• Perform biochemical assays, including Western blotting, immunofluorescence, and flow cytometry.
• Conduct molecular biology techniques, such as RNA extraction, cDNA synthesis, and RT-qPCR.
• Support or conduct CRISPR gene editing experiments and lentivirus production/transduction.
• Maintain detailed documentation of experimental protocols, procedures, and results.
• Engage in regular team meetings and ensure open communication with team members and supervisors.
• Potential for authorship in scientific publications depending on contributions.

Requirements:

• Bachelor's or master's degree in Biological Sciences, Molecular Biology, Neuroscience, Biochemistry, or a related field.
• BS with 2 3 years of tissue culture experience or MS with at least 1 year of relevant lab experience.
• Must have prior training in standard wet lab practices and basic laboratory instrumentation.
• Advanced cell culture techniques (maintenance, passaging, cryopreservation).
• Proficiency in biochemical assays (Western blot, immunofluorescence, flow cytometry).
• Proficiency in molecular biology techniques (RNA extraction, cDNA synthesis, RT-qPCR).
• Strong documentation, organizational, and communication skills.
• Experience with human stem cell reprogramming, maintenance, and differentiation.
• Hands-on experience with CRISPR gene editing technologies.
• Lentiviral production and transduction experience.
• Advanced Cell Culture Expertise: maintenance, freezing, thawing, and passaging.
• Biochemistry Techniques: Western blot, Immunofluorescence, flow cytometry.
• Molecular Biology Skills: RNA extraction, cDNA synthesis, RT-QPCR.
• Gene editing using CRISPR technology.
• Wet Lab & Equipment Proficiency.

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