Website Nesco Resource, LLC
Nesco Resource, LLC
Company : Nesco Resource, LLC
Job Title: Scientist, Cell-Based Assays
Requirements:
• BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5+ years, Masters with 3+ years and PHD with 1+ year of industry or equivalent lab experience.
• Proficiency in mammalian cell culture and 384-well assay workflows
• Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)
• Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)
• Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment
Nice to Have:
• Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z', RESUME, S/B)
• Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools
• Hands-on experience with Echo acoustic dispensing.
Note:
• Onsite assignment @ Rahway,NJ.
• 100% Lab based role.
Overview:
We're seeking a hands-on scientist to execute high-throughput cell-based assays in 384-well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high-quality EC50 results for discovery programs.
Key Responsibilities:
• Maintain, expand, and plate mammalian cell lines for 384-well assays
• Set up and execute assays with compound dispensing via Labcyte/Beckman Echo
• Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR-FRET, fluorescence)
• Generate dose–response curves (4-parameter logistic fits) and report EC50/IC50 with QC
• Troubleshoot assay performance, optimize Z' factors, and document SOPs/results
• Collaborate with discovery teams to prioritize studies and timelines
Additional responsibilities may include setting up western blot assays and learning how to run in-cell westerns